Journal: Oncotarget

Article Title: Upregulation of calprotectin in mild IgE-mediated ovalbumin hypersensitivity

doi: 10.18632/oncotarget.16954

Figure Lengend Snippet: Body weight ( A ) and length ( B ) were monitored weekly from the beginning of the study to the end. There was no significant difference between two groups from days 7 to days 42 (day 7 to 35, n = 5/group; day 42, n = 15/group, P all > 0.05).

Article Snippet: Immunohistochemistry was performed using standard protocols with the following antibodies: IL-1β, (1:100, both from Origene, Inc., Rockville, MD, USA); TNF-α, S100A8, S100A9 (1:200, Abcam, Inc., Cambridge, MA, USA); Toll-like receptor 4 (TLR4), S100A8/A9 (1:200, NOVUSbio, Inc., Littleton, CO, USA); nuclear factor kappa B (NF-κB) (1:200, Cell Signaling Technology, Inc., Danvers, MA, USA).

Techniques:

Journal: Oncotarget

Article Title: Upregulation of calprotectin in mild IgE-mediated ovalbumin hypersensitivity

doi: 10.18632/oncotarget.16954

Figure Lengend Snippet: PCR was used to detect the mRNA expression of TLR4, NF-κB, TNF-α, IL-1β, and IL-6. ( A , B , C , D , and E ) show the expression of TLR4, NF-κB, TNF-α, IL-1β, and IL-6 in jejunum; ( F , G , H , I and J ) show the expression of TLR4, NF-κB, TNF-α, IL-1β, and IL-6 mRNA in liver. * P < 0.05 ; # P < 0.01. ( K ) shows the expression of TLR4, NF-κB, TNF-α, and IL-1β. from left to right are control group, hypersensitivity groups from day 7 to day 42; and from top to bottom, expression of TLR4, NF-κB, TNF-α, and IL-1β in jejunum.

Article Snippet: Immunohistochemistry was performed using standard protocols with the following antibodies: IL-1β, (1:100, both from Origene, Inc., Rockville, MD, USA); TNF-α, S100A8, S100A9 (1:200, Abcam, Inc., Cambridge, MA, USA); Toll-like receptor 4 (TLR4), S100A8/A9 (1:200, NOVUSbio, Inc., Littleton, CO, USA); nuclear factor kappa B (NF-κB) (1:200, Cell Signaling Technology, Inc., Danvers, MA, USA).

Techniques: Expressing, Control